How would you carry out the following dilutions?
Dilute 0.1M stock solution to give 1 litre of 100µM
Notes:
Hint 1
Hint 1: Step 1: work out the quantity of substance required (volume x concentration) Step 2: work out what volume of stock solution contains this quantity Step 3:The volume of distilled water needed is the volume of the final solution minus the volume calculated in Step 2.
Hint 2
Hint 2: Equation Method (logic is safer!) S=Conc of stock (M) V=Vol of final solution (Litres) C=Conc of final soln. (M) Vs=vol of stock needed (Litres) Vw=vol of water needed Vs=VC/S; Vw=V-V